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Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
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Fluorescence resonance energy transfer using DNA-templated copper nanoparticles for ratiometric detection of
1Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, Republic of Korea. akdong486@konkuk.ac.kr.
The Analyst
|February 19, 2021
Abstract:
We examined the effectiveness of a ratiometric method using DNA-templated copper nanoparticles, which can function as a probe for fluorescence resonance energy transfer. This method in combination with PCR successfully detected the target microRNA, which corresponded well with the results obtained by quantitative reverse transcription PCR.

