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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Competitive Genomic Screens of Barcoded Yeast Libraries

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Array-based dynamic allele specific hybridization (Array-DASH): Optimization-free microarray processing for multiple

Spencer J Gibson¹, Nathalie Zahra², Peter J Freeman³

¹Department of Genetics, Adrian Building, University of Leicester, University Road, Leicester, Leicestershire, LE17RH, UK.

Analytical Biochemistry

|February 19, 2021

Summary

This summary is machine-generated.

This study introduces a dynamic microarray protocol for precise DNA analysis. This method allows for accurate DNA re-sequencing, fingerprinting, and genotyping with high-fidelity detection of sequence variations.

Keywords:

Genotyping HIV Hypericum Microarray Re-sequencing Semi-quantitative

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Last Updated: Nov 16, 2025

Competitive Genomic Screens of Barcoded Yeast Libraries

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Infinium Assay for Large-scale SNP Genotyping Applications

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Published on: November 19, 2013

Area of Science:

Molecular Biology
Genomics
Biotechnology

Background:

Microarray technology is crucial for high-throughput genetic analysis.
Accurate and sensitive DNA analysis is essential for diagnostics and research.
Current methods may have limitations in speed, accuracy, or cost-effectiveness for certain applications.

Purpose of the Study:

To present a novel dynamic microarray protocol for DNA analysis.
To demonstrate the protocol's capability for accurate DNA re-sequencing, fingerprinting, and genotyping.
To assess the potential for detecting low-frequency genetic variations.

Main Methods:

Utilizing a dynamic microarray protocol with surface-bound probes and gradual temperature changes.
Employing real-time fluorescence tracking to monitor target molecule denaturation.
Performing melt-curve analysis to identify the probe with the highest melting temperature, indicating sequence complementarity.

Main Results:

Achieved >99% accuracy in DNA re-sequencing.
Demonstrated a potential single nucleotide polymorphism (SNP) detection rate as low as 1%.
Successfully performed simultaneous re-sequencing and genotyping with a reliable detection level of 5% for Hypericum ribosomal ITS regions and HIV genomes.

Conclusions:

The dynamic microarray protocol offers accurate and semi-quantitative DNA analysis.
The technique shows promise for rapid sequence interrogation and applications like infectious disease diagnostics (e.g., COVID-19).
Further development towards a commercial platform and software could facilitate widespread adoption in scientific research.