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Updated: Nov 16, 2025
Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
Published on: November 13, 2017
A sensitive sandwich ELISA using a modified biotin-streptavidin amplified system for histamine detection in fish,
Fanfan Yang1, Long Xu1, Alberto C P Dias1
1Centre of Molecular and Environmental Biology, University of Minho, Department of Biology, Campus de Gualtar, 4710-057 Braga, Portugal.
Abstract:
Histamine poisoning from seafood is a significant public health and safety concern. To detect histamine sensitively and accurately, a novel competitive sandwich immunoassay using a modified biotin-streptavidin system coupling with polylysine was developed. Using this strategy, a sandwich ELISA with an IC50 value of 112.8 ng mL-1 and a broad linear range of 11.7-1500 ng mL-1 with a correlation coefficient of 0.9942 was validated. Without any sample derivatization procedure, the recovery of histamine ranged from 80.19% to 108.3% with a coefficient of variation of 1.43-11.7% in tuna, prawn and crab. The sandwich ELISA had a detectionlimit of 5.86 ng mL-1, which was 15-fold lower than an indirect competitive ELISA (ic-ELISA). This simple, sensitive and accurate method can be applied to detect histamine in routine seafood samples.
