Mutation analysis for 25 Y-STR markers in Japanese population

Area of Science:

• Forensic Genetics
• Population Genetics
• Human Genetics

Background:

• Y-chromosome short tandem repeat (Y-STR) markers are crucial for forensic identification and paternity testing.
• Understanding Y-STR mutation rates is essential for accurate genetic analysis in specific populations.

Purpose of the Study:

• To evaluate the efficacy of 25 Y-STR markers for paternity testing and forensic identification in the Japanese population.
• To determine Y-STR mutation rates for each locus using Japanese father-son pairs.

Main Methods:

• DNA samples from 213 Japanese father-son pairs were analyzed.
• Twenty-five Y-STR markers were amplified using the Yfiler™ Plus PCR amplification kit.
• Y-STR mutation rates were calculated for each locus.

Main Results:

• Mutation rates for most Y-STR markers were below 1.0 × 10⁻².
• The mutation rates for DYS576 and DYS458 were also determined.
• Six previously identified rapidly mutating Y-STR markers were included in the analysis.

Conclusions:

• The 25 Y-STR markers analyzed demonstrate potential utility for forensic identification within the Japanese population.
• The calculated mutation rates support the application of these markers in genetic analyses.
• Further research may validate these findings for broader forensic applications.