Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

15.6K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
15.6K
Genetic Screens02:46

Genetic Screens

5.3K
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
5.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CELLISA - a cell-cell binding assay for evaluation of nanovesicle targeting proteins.

bioRxiv : the preprint server for biology·2026
Same author

Distinguishing Pseudotransduction and True Transduction Enables Characterization and Bioengineering of Extracellular Vesicle-Adeno-Associated Virus Vectors.

Journal of extracellular vesicles·2026
Same author

GCAD: A Computational Framework for Mammalian Genetic Program Computer-Aided Design.

ACS synthetic biology·2026
Same author

Site-specific genome engineering of primary human natural killer cells for programmable anti-tumor function.

bioRxiv : the preprint server for biology·2025
Same author

Integrated epigenetic and genetic programming of primary human T cells.

Nature biotechnology·2025
Same author

GCAD: a Computational Framework for Mammalian Genetic Program Computer-Aided Design.

bioRxiv : the preprint server for biology·2025
Same journal

Taphonomic analysis at Liang Bua reveals the behavioral and technological capabilities of <i>Homo floresiensis</i>.

Science advances·2026
Same journal

Targeting granule initiation and amyloplast structure to create giant starch granules in wheat.

Science advances·2026
Same journal

A meta-analysis of carbon losses and gains from tropical moist forest degradation and regeneration.

Science advances·2026
Same journal

Ancient DNA reveals elite dynastic rule among Iron Age Eurasian Steppe nomads.

Science advances·2026
Same journal

Targeting astrocytic Dp71 attenuates BBB disruption after traumatic brain injury through WTAP-associated m<sup>6</sup>A regulation of MMP2.

Science advances·2026
Same journal

Pancreatic α cells are required for nutrient homeostasis by regulating dynamic β cell networks in islets.

Science advances·2026
See all related articles

Related Experiment Video

Updated: Nov 16, 2025

Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids
10:49

Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids

Published on: February 3, 2023

3.2K

Model-guided design of mammalian genetic programs.

J J Muldoon1,2, V Kandula3, M Hong2

  • 1Interdisciplinary Biological Sciences Program, Northwestern University, Evanston, IL 60208, USA.

Science Advances
|February 20, 2021
PubMed
Summary
This summary is machine-generated.

We developed a new method for designing genetic programs in mammalian cells. This approach uses high-performance genetic parts and computational models for predictable cellular engineering.

More Related Videos

Somatic Genome-Engineered Mouse Models Using In Vivo Microinjection and Electroporation
08:06

Somatic Genome-Engineered Mouse Models Using In Vivo Microinjection and Electroporation

Published on: May 5, 2023

2.2K
Mouse Genome Engineering Using Designer Nucleases
12:04

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

29.1K

Related Experiment Videos

Last Updated: Nov 16, 2025

Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids
10:49

Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids

Published on: February 3, 2023

3.2K
Somatic Genome-Engineered Mouse Models Using In Vivo Microinjection and Electroporation
08:06

Somatic Genome-Engineered Mouse Models Using In Vivo Microinjection and Electroporation

Published on: May 5, 2023

2.2K
Mouse Genome Engineering Using Designer Nucleases
12:04

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

29.1K

Area of Science:

  • Synthetic biology
  • Mammalian cell engineering
  • Genetic circuit design

Background:

  • Engineering mammalian cells for complex functions is challenging.
  • Current methods lack systematic approaches for predictable genetic programming.

Purpose of the Study:

  • To develop a method for accurate genetic program design in mammalian cells.
  • To enable predictable engineering of complex cellular functions.

Main Methods:

  • Developed multifunctional proteins with transcriptional and posttranslational control.
  • Validated computational models for genetic mechanisms.
  • Implemented digital and analog processing within genetic circuits.
  • Integrated genetic circuits with sensors for multi-input evaluation.

Main Results:

  • Demonstrated functional modularity and compositional versatility of genetic parts.
  • Enabled multiple synonymous genetic programs for a single design objective.
  • Successfully predicted and achieved desired mammalian cellular functions.

Conclusions:

  • The developed method empowers bioengineers to design complex cellular functions predictably.
  • This approach enhances the systematic engineering of mammalian cells.
  • Advances in genetic programming offer significant technological and translational applications.