Related Experiment Video

Updated: Aug 25, 2026

In vitro Investigation of the MexAB Efflux Pump From Pseudomonas aeruginosa
13:40

In vitro Investigation of the MexAB Efflux Pump From Pseudomonas aeruginosa

Published on: February 17, 2014

Proline transport activity in Escherichia coli membrane vesicles of different buoyant densities

H van Heerikhuizen, M Boekhout, B Witholt

    Biochimica Et Biophysica Acta
    |November 1, 1977
    PubMed

    More Related Videos

    A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl- Channels and Transporters07:47

    A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl- Channels and Transporters

    Published on: April 20, 2015

    Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles13:16

    Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles

    Published on: December 31, 2019

    Related Concept Videos

    Protein Diffusion in the Membrane01:24

    Protein Diffusion in the Membrane

    Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
    The Significance of Membrane Transport01:44

    The Significance of Membrane Transport

    The transport of solutes across the cell membrane is essential for metabolic processes, like maintaining cell size and volume, generating the action potential, exchanging nutrients and gases, etc. Membrane transport can be either passive or active. It can be simple diffusion, facilitated, or mediated transport aided by transport proteins such as transporters and channels.
    Transporters facilitate either an active or passive movement of solutes. They can allow a single-molecule transport down its...
    Overview of Protein Sorting and Transport01:45

    Overview of Protein Sorting and Transport

    Eukaryotic cells have different membrane-bound organelles with distinct protein requirements. The process by which proteins are targeted to a specific organelle is called protein sorting.
    Protein sorting can be of two types: signal-based sorting and vesicle-based trafficking. In signal-based sorting, specific amino acid sequences called sorting signals target proteins to the proper location inside the cell either via gated transport or by protein translocation.  In gated transport, folded...
    COP Coated Vesicles00:59

    COP Coated Vesicles

    Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of different...