Phylogeny and classification of Odonata using targeted genomics.

Area of Science:

• * Entomology
• * Evolutionary Biology
• * Phylogenomics

Background:

• * Dragonflies and damselflies (Odonata) offer unique comparative research potential due to well-understood taxonomy and ecology.
• * Previous large-scale phylogenies were lacking, hindering evolutionary studies.
• * High-throughput data was needed to connect taxonomic and ecological perspectives.

Purpose of the Study:

• * To construct a large-scale phylogeny for Odonata using anchored hybrid enrichment.
• * To propose an updated classification for Odonata based on phylogenomic reconstruction.
• * To investigate evolutionary scenarios like incomplete lineage sorting and introgression.

Main Methods:

• * Anchored hybrid enrichment sequencing.
• * Analysis of 478 target loci from 136 species representing 46 families.
• * Quartet sampling approach to explore evolutionary scenarios.

Main Results:

• * Monophyly of all three suborders (Anisoptera, Anisozygoptera, Zygoptera) was recovered.
• * The phylogeny's backbone shows high support, but some portions remain unresolved.
• * Five new families and two reinstated families were proposed, alongside an updated classification.

Conclusions:

• * The study provides a robust phylogenomic framework for Odonata.
• * Incomplete lineage sorting and introgression may have influenced the evolutionary history.
• * An updated classification is proposed, highlighting areas for future taxonomic research.