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Updated: Nov 16, 2025

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
Research progress on enhancing the performance of autotrophic nitrogen removal systems using microbial immobilization
1School of Resource and Environmental Engineering, Wuhan University of Technology, Wuhan, Hubei 430070, China.
Abstract:
The autotrophic nitrogen removal process has great potential to be applied to the biological removal of nitrogen from wastewater, but its application is hindered by its unstable operation under adverse environmental conditions, such as those presented by low temperatures, high organic matter concentrations, or the presence of toxic substances. Granules and microbial entrapment technology can effectively retain and enrich microbial assemblages in reactors to improve operating efficiency and reactor stability. The carriers can also protect the reactor's internal microorganisms from interference from the external environment. This article critically reviews the existing literature on autotrophic nitrogen removal systems using immobilization technology. We focus our discussion on the natural aggregation process (granulation) and entrapment technology. The selection of carrier materials and entrapment methods are identified and described in detail and the mechanisms through which entrapment technology protects microorganisms are analyzed. This review will provide a better understanding of the mechanisms through which immobilization operates and the prospects for immobilization technology to be applied in autotrophic nitrogen removal systems.
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