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Updated: Nov 16, 2025

Targeting Biofilm Associated Staphylococcus aureus Using Resazurin Based Drug-susceptibility Assay
Published on: May 5, 2016
A dynamic resazurin microassay allowing accurate quantification of cells and suitable for acid-forming bacteria
Maritxu Labadie1, Irina Randrianjatovo-Gbalou2, Meriem Zaidi-Ait-Salem1
1Université de Toulouse, UPS, IUT Paul Sabatier, LBAE EA 4565 (Laboratoire de Biotechnologies Agroalimentaire et Environnementale), 24 rue d'Embaquès, Auch F-32000, France.
Abstract:
A resazurin micro-assay was developed to quantify acidifying bacteria. The resorufin fluorescent signal was measured over time and the determined time to reach the max slope (TMS) was plotted against CFU (colony forming unit) counts. This dynamic assay enabled to quantify nine lactic acid bacteria and a Bacillus licheniformis strain despite the increasing acidity of the medium.
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