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Enhancement of anti-phospholipid antibody activity by Tween 20

C H Ming1, Y S Fan

  • 1Department of Physiology, Faculty of Medicine, University of Malaya, Kuala Lumpur.

Addition of the non-ionic detergent Tween 20 to the serum diluent enhances anti-cardiolipin binding reactivity in an ELISA system. Maximal enhancement was obtained using a concentration of 0.05% Tween 20 in the diluent. Non-specific interactions were also considerably reduced.

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