Bacterial mediated silver nanoparticles and their efficacy against MRSA

Area of Science:

• Biotechnology
• Nanotechnology
• Microbiology

Background:

• Bacterial-mediated synthesis of silver nanoparticles (AgNPs) offers an eco-friendly alternative for pathogen eradication.
• Nosocomial infections, particularly those caused by Methicillin-resistant Staphylococcus aureus (MRSA), pose a significant global health challenge.
• There is a need for novel antimicrobial agents and sustainable synthesis methods.

Purpose of the Study:

• To biologically synthesize silver nanoparticles using indigenously isolated soil bacteria.
• To optimize the synthesis process for maximum AgNP production.
• To evaluate the antibacterial efficacy of synthesized AgNPs against MRSA.

Main Methods:

• Extracellular synthesis of AgNPs using cell-free filtrate of bacterial strains.
• Optimization of synthesis conditions for enhanced nanoparticle yield.
• Characterization of AgNPs using UV-Vis spectroscopy and Transmission Electron Microscopy (TEM).
• In-vitro antibacterial activity testing against MRSA.

Main Results:

• Characterization confirmed the presence of AgNPs with a plasmon resonance peak between 420-450nm.
• TEM analysis revealed AgNPs with sizes ranging from 10-60nm, predominantly spherical.
• Synthesized AgNPs exhibited significant in-vitro antibacterial activity against MRSA.

Conclusions:

• Bacterial synthesis provides a green and sustainable route for AgNP production.
• The synthesized AgNPs show potential as an effective antimicrobial agent against MRSA.
• This approach can contribute to managing nosocomial infections and offers a novel application in Southern Punjab, Pakistan.