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Related Experiment Videos

Negative inotropic effect of platelet-activating factor: association with a decrease in intracellular sodium

D A Robertson1, D Y Wang, C O Lee

  • 1Department of Pharmacology, Cornell University Medical College, New York, New York.

The Journal of Pharmacology and Experimental Therapeutics
|April 1, 1988
PubMed
Summary
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Platelet-activating factor (PAF) reduces heart muscle contraction by decreasing intracellular sodium activity (aiNa). This reduction in sodium may lead to calcium loss via sodium-calcium exchange, impacting cardiac contractility.

Area of Science:

  • Cardiovascular Physiology
  • Cellular Electrophysiology

Background:

  • Platelet-activating factor (PAF) is known to cause a negative inotropic effect on the heart.
  • The precise mechanism behind PAF-induced myocardial contractility reduction remains unclear, with conflicting reports on calcium influx.

Purpose of the Study:

  • To investigate the effect of Platelet-activating factor (PAF) on intracellular sodium activity (aiNa) in cardiac muscle.
  • To elucidate the role of intracellular sodium in the negative inotropic effect of PAF.

Main Methods:

  • Isolated guinea pig right ventricular papillary muscles were used.
  • Contractile responses, transmembrane action potential, and intracellular sodium activity (aiNa) were measured.
  • The effect of PAF and its antagonist CV-3988 were assessed.

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Main Results:

  • Platelet-activating factor (PAF) did not alter slow inward calcium current.
  • PAF induced a negative inotropic effect, shortened action potential duration, and decreased intracellular sodium activity (aiNa).
  • The PAF antagonist CV-3988 blocked these electro-mechanical responses.

Conclusions:

  • The negative inotropic effect of Platelet-activating factor (PAF) is associated with a reduction in intracellular sodium activity (aiNa).
  • Decreased aiNa may lead to calcium extrusion via Na+/Ca++ exchange, reducing contractility.
  • PAF-induced action potential duration shortening may result from decreased sodium influx.