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A new biflavonoids from Aster tataricus induced non-apoptotic cell death in A549 cells
Ting Chen1, Peng Yang2, Hai-Jun Chen2
1Department of Pharmacy, Guizhou Health Vocational College, Tongren, China.
Abstract:
A new biflavonoids, (2R,2''R)-7-O-methyl-2,3,2'',3''-tetrahydrorobustaflavone (1), along with five known flavonoids (2-6) were isolated from the MeOH extract of Aster tataricus. Among them, compounds 1-2 were the C-3'-C-6'' type biflavonoids obtained from the genus Aster for the first time. The structures and absolute configurations of compound 1 was confirmed based on extensive spectroscopic and circular dichroism analyses. Compound 1 exhibited moderate cytotoxicity against seven human cancer A549, HepG2, PC3, DU145, MCF-7, LOVO and NCI-H1975 cell lines. Compound 1 remarkably inhibited the proliferation of A549 cancer cells with IC50 value of 5.4 μM. Further preliminary pharmacological study, 1 induces A549 cell death by non-apoptotic forms through flow cytometry and cell scratch assay data.
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