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Published on: February 21, 2018
Inhibition of TGF-β1 on Gli2 expression was promoted by TNF-α in primary leukemia cells
Zhe Li1, Shudan Mao1, Ning Zhang1
1Department of Hematology, The First Affiliated Hospital of Jinzhou Medical University, Liaoning, P. R. China.
Purpose:
Hedgehog (Hh) signaling pathway regulates a variety of tumors-related diseases including leukemia. Whether inhibition of TGF-β1 on Gli2 expression is promoted by TNF-α in primary leukemia cells remains to be determined.
Methods:
Primary leukemia cells were treated with TGF-β1, TNF-α or SIS3 at different concentrations. Gli2 expression was detected by quantitative real-time PCR and western blot analyses.
Results:
We found that TGF-β significantly decreased Gli2 expression, and co-treatment with TNF-αfurther decreased Gli2 expression in primary leukemia cells. TNF-α can increased TGF-βRI and TGF-βRII protein expression in primary leukemia cells, while SIS3 inhibited the effect of TGF-β.
Conclusion:
Our results suggest that Gli2 expression in primary leukemia cells is induced by TGF-β in a Smad3-dependent manner, and independent of Hh receptor signaling.
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