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Updated: Nov 16, 2025
Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Exploring the Characteristics of Intestinal Microbiota in Hematologic Malignancy Patients via 16s rDNA
Background:
Malignant hematopathy is an important branch of malignant tumors, with high mortality and malignancy. The purpose of this study was to investigate the relationship between intestinal microecology and diseases by observing patients with newly diagnosed hematologic malignancy.
Methods:
In this study a total of 23 stool samples were collected and analyzed, 13 of which were stool samples from newly diagnosed patients with malignant blood system diseases, and 10 were healthy individual controls. The characteristics of the intestinal flora were analyzed through 16s rDNA technology in the next-generation sequenc-ing (NGS).
Results:
Bacteroidetes and Firmicutes accounted for the major abundance of the intestinal microecology in both patients with hematological malignancies and healthy controls, whereas the abundance of Bacteroidetes in the patient group was lower than that in healthy controls. Linear discriminant analysis (LDA) in LEfSa was used to search for landmark species, suggesting that Erysipelotrichi, Erysipelotrichales, and Erysipelotrichaceae could be considered as markers for patients with hematological malignant diseases. Butyricicoccus pullicaecorum, Bacteroides plebeius, and Collinsella aerofaciens also contribute to potential values as markers for intestinal flora in hematological malignant patients.
Conclusions:
Patients with hematologic malignancies have altered intestinal flora structure compared with healthy individuals, which can provide new ideas for the treatment of hematologic malignancies.
