Arenibacter arenosicollis sp. nov., isolated from a sand dune

A Gram-stain-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterial strain, designated BSSL-BM3^T, was isolated from sand collected from a dune near the Yellow Sea, Republic of Korea, and subjected to a polyphasic taxonomic study. The neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain BSSL-BM3^T fell within the clade comprising the type strains of Arenibacter species. Strain BSSL-BM3^T exhibited 16S rRNA gene sequence similarity values of 98.0-99.0 % to the type strains of Arenibacter catalasegens, Arenibacter hampyeongensis, Arenibacter echinorum, Arenibacter palladensis and Arenibacter troitsensis and of 94.2-96.7 % to the type strains of the other Arenibacter species. The averagenucleotide identity and digitalDNA-DNA hybridization values between strain BSSL-BM3^T and the type strains of A. catalasegens, A. hampyeongensis, A. echinorum, A. palladensis and A. troitsensis were 82.2-88.8 % and 25.0-36.5 %, respectively. The DNA G+C content of strain BSSL-BM3^T from genomic sequence data was 38.75 mol%. Strain BSSL-BM3^T contained MK-6 as the predominant menaquinone and iso-C_17 : 0 3-OH, summed feature 3 (C_16 : 1  ω7c and/or C_16 : 1  ω6c) and iso-C_15 : 1 G as the major fatty acids. The major polar lipids of strain BSSL-BM3^T were phosphatidylethanolamine and two unidentified lipids. Distinguishing phenotypic properties, along with the phylogenetic and genetic distinctiveness, revealed that strain BSSL-BM3^T is separated from recognized Arenibacter species. On the basis of the data presented here, strain BSSL-BM3^T is considered to represent a novel species of the genus Arenibacter, for which the name Arenibacter arenosicollis sp. nov. is proposed. The type strain is BSSL-BM3^T (=KACC 21632^T=NBRC 114502^T).