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LncRNA Rian ameliorates sevoflurane anesthesia-induced cognitive dysfunction through regulation of miR-143-3p/LIMK1
Yang Yu1, Wei Zhang2, Dengyan Zhu3
1Department of Anesthesiology, Pain and Perioperative Medicine, The First Affiliated Hospital of Zhengzhou University, No. 1, Jianshe East Road, Erqi District, Zhengzhou, 450052, Henan, China.
Human Cell
|February 22, 2021
Summary
Long non-coding RNA Rian protects against sevoflurane-induced cognitive dysfunction by regulating the miR-143-3p/LIMK1 pathway, offering a potential therapeutic target for postoperative cognitive dysfunction.
Area of Science:
- Neuroscience
- Molecular Biology
- Anesthesiology
Background:
- Sevoflurane anesthesia can cause neurotoxicity, leading to postoperative cognitive dysfunction (POCD).
- Long non-coding RNAs (lncRNAs) play roles in nervous system diseases, but their involvement in sevoflurane-induced cognitive impairment is unclear.
Purpose of the Study:
- To investigate the role and mechanism of lncRNA Rian (RNA imprinted and accumulated in nucleus) in sevoflurane anesthesia-induced cognitive dysfunction.
Main Methods:
- Mice were subjected to sevoflurane anesthesia, and cognitive function, neuronal damage, and apoptosis were assessed.
- Adenovirus-mediated overexpression of Rian was used to evaluate its neuroprotective effects in vivo and in primary hippocampal neurons.
- The interaction between Rian, miR-143-3p, and LIMK1 (Lim kinase 1) was investigated to elucidate the underlying mechanism.
Main Results:
- Sevoflurane anesthesia induced cognitive impairments, neuronal damage, and apoptosis in mice.
- Overexpression of Rian ameliorated sevoflurane-induced neuronal damage, apoptosis, and cognitive deficits.
- Rian protected neurons by binding to miR-143-3p, thereby decreasing LIMK1 expression, and manipulating LIMK1 levels affected neuronal viability and apoptosis.
Conclusions:
- lncRNA Rian exerts neuroprotective effects against sevoflurane-induced cognitive dysfunction.
- The mechanism involves the Rian/miR-143-3p/LIMK1 axis.
- Rian represents a potential therapeutic target for preventing sevoflurane-induced cognitive dysfunction.
