Related Experiment Video
Updated: Nov 16, 2025
10:37
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Published on: November 15, 2017
12.4K
Multi-Omic Single-Shot Technology for Integrated Proteome and Lipidome Analysis
Yuchen He1, Edrees H Rashan2, Vanessa Linke1
1Department of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States.
Analytical Chemistry
|February 22, 2021
Summary
This study introduces multi-omic single-shot technology (MOST) for simultaneous protein and lipid analysis using mass spectrometry. MOST simplifies workflows, enabling robust, reproducible, and integrated multi-omics data acquisition.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Systems Biology
Background:
- Mass spectrometry (MS) is crucial for analyzing proteomes, lipidomes, and metabolomes.
- Integrating multi-omic data is essential for understanding complex biological networks.
- Current MS methods often require specialized labs, limiting simultaneous analysis of different molecular classes.
Purpose of the Study:
- To develop a streamlined technology for simultaneous analysis of multiple molecular classes.
- To overcome the limitations of specialized MS workflows.
- To enable integrated protein and lipid analysis in a single experiment.
Main Methods:
- Development of multi-omic single-shot technology (MOST).
- Utilizes a single column and LC-MS instrument for broad and deep coverage.
- Simplified workflow for simultaneous extraction and analysis of diverse biomolecules.
Main Results:
- MOST demonstrated high robustness and reproducibility.
- Successfully applied to Saccharomyces cerevisiae, analyzing 20 conditions.
- Identified 2842 protein groups and 325 lipids, revealing potential molecular relationships.
Conclusions:
- MOST enables simultaneous, comprehensive analysis of proteins and lipids.
- This technology simplifies multi-omics integration and data acquisition.
- MOST advances the field of integrated multi-omics research.
Related Concept Videos
01:33Proteomics
8.8K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
8.8K
01:33Peptide Identification Using Tandem Mass Spectrometry
7.6K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
7.6K