Endocrine Disruptor Exposure Causes Infochemical Dysregulation and an Ecological Cascade from Zooplankton to Algae

Endocrine disruption is intimately linked to controlling the population of pollutant-exposed organisms through reproduction and development dysregulation. This study investigated how endocrine disruption in a predator organism could affect prey species biology through infochemical communication. Daphnia magna and Chlorella vulgaris were chosen as model prey and predator planktons, respectively, and fenoxycarb was used for disrupting the endocrine system of D. magna. Hormones as well as endo- and exometabolomes were extracted from daphnids and algal cells and their culture media and analyzed using liquid chromatography with tandem mass spectrometry. Biomolecular perturbations of D. magna under impaired offspring production and hormone dysregulation were observed. Differential biomolecular responses of the prey C. vulgaris, indicating changes in methylation and infochemical communication, were subsequently observed under the exposure to predator culture media, containing infochemicals released from the reproducibly normal and abnormal D. magna, as results of fenoxycarb exposure. The observed cross-species transfer of the endocrine disruption consequences, initiated from D. magna, and mediated through infochemical communication, demonstrates a novel discovery and emphasizes the broader ecological risk of endocrine disruptors beyond reproduction disruption in target organisms.