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Updated: Nov 16, 2025

The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes

The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes

Published on: May 25, 2018

Quantitative profiling of protease specificity.

Boris I Ratnikov¹, Piotr Cieplak¹, Albert G Remacle¹

¹Sanford Burnham Prebys Medical Discovery Institute, La Jolla, California, United States of America.

Plos Computational Biology

|February 22, 2021

Summary

This summary is machine-generated.

Researchers defined a protease

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Last Updated: Nov 16, 2025

The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes

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Published on: May 25, 2018

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Detection of Protease Activity by Fluorescent Peptide Zymography

Detection of Protease Activity by Fluorescent Peptide Zymography

Published on: January 20, 2019

Area of Science:

Biochemistry
Enzymology
Molecular Biology

Background:

Proteases are crucial enzymes in physiological and pathological processes.
Understanding protease specificity is vital for elucidating enzyme function.
Existing methods often lack the comprehensive scope to fully define protease specificity.

Purpose of the Study:

To introduce the
selectome
concept for defining protease specificity.
To quantitatively measure protease substrate specificity.
To differentiate substrate selectivity between closely related proteases.

Main Methods:

Substrate phage display coupled with Next Generation Sequencing.
Information theory-based data analysis.
Development of a quantitative measure for substrate specificity.

Main Results:

Applied the selectome concept to Matrixin family members MMP-2 and MMP-9.
Derived a quantitative measure accounting for substrate number and catalytic efficiency.
Facilitated the elucidation of substrate selectivity between related proteases.

Conclusions:

The selectome concept and quantitative measure enhance understanding of protease specificity.
Provides a basis for developing selective activity probes for proteases.
Aids in distinguishing targeted proteolysis from bystander events.