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Single-Molecule Imaging of Nuclear Transport
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Physical modeling of multivalent interactions in the nuclear pore complex.

Luke K Davis1, Anđela Šarić2, Bart W Hoogenboom1

  • 1Department of Physics and Astronomy; Institute for the Physics of Living Systems; London Centre for Nanotechnology, University College London, London, United Kingdom.

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|February 22, 2021
PubMed
Summary

Heterogeneity in intrinsically disordered FG Nups and nuclear transport receptors (NTRs) significantly impacts binding kinetics, not just equilibrium. New models reveal how sequence and surface variations influence transport selectivity within the nuclear pore complex.

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Area of Science:

  • Molecular Biology
  • Biophysics
  • Cell Biology

Background:

  • The nuclear pore complex regulates transport via interactions between FG Nups and NTRs.
  • Previous minimal models simplified FG Nups and NTRs as homogeneous, neglecting sequence and surface heterogeneities.

Purpose of the Study:

  • To develop and validate computational and analytical models incorporating FG Nup and NTR heterogeneity.
  • To investigate the role of this heterogeneity in binding equilibria and kinetics.

Main Methods:

  • Development of novel computational and analytical models accounting for heterogeneous FG Nup sequences and NTR surfaces.
  • Comparison of model predictions with experimental single-molecule interaction data.

Main Results:

  • Heterogeneity in FG Nups and NTRs significantly affects binding and unbinding kinetics, more so than equilibrium properties.
  • Models predict dependencies of binding equilibria and kinetics on the distribution of cohesive blocks and binding pockets, highlighting multivalency's role.
  • Single-molecule binding kinetics minimally influence NTR diffusion in FG-Nup-like polymer melts.

Conclusions:

  • Explicitly modeling heterogeneity in FG Nups and NTRs is crucial for accurately describing nuclear transport.
  • Sequence and surface heterogeneities are key determinants of transport dynamics and selectivity.
  • The findings provide a more refined understanding of molecular interactions within the nuclear pore complex.