Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Craspase Protease Activation Is Sensitive to Oncogenic Single-Nucleotide RNA Mismatches.

ACS chemical biology·2026
Same author

A defense-like route to gene transfer agent release.

Cell chemical biology·2026
Same author

YprA-family helicases provide the missing link between diverse prokaryotic immune systems.

Cell host & microbe·2026
Same author

Antiphage defence systems Druantia III and Zorya II synergise via shared DNA intermediates in a phage-specific manner.

bioRxiv : the preprint server for biology·2026
Same author

DepoCatalog: mapping diversity of 129 recombinantly produced Klebsiella phage depolymerases.

Nature communications·2026
Same author

Tmn blocks phage spread via plasmolysis and triggers synergistic defence responses.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Nov 16, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

1.7K

CRISPR-based DNA and RNA detection with liquid-liquid phase separation.

Willem Kasper Spoelstra1, Jeroen M Jacques1, Rodrigo Gonzalez-Linares2

  • 1Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, Delft, the Netherlands.

Biophysical Journal
|February 22, 2021
PubMed
Summary

This study introduces a novel, label-free method for detecting specific DNA and RNA sequences using CRISPR-Cas enzymes and liquid-liquid phase separation (LLPS). This technique enables naked-eye detection of nucleic acids, offering a simple and cost-effective diagnostic tool.

More Related Videos

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
09:03

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

3.0K
DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
04:17

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

Published on: May 10, 2024

1.2K

Related Experiment Videos

Last Updated: Nov 16, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

1.7K
Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
09:03

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

3.0K
DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
04:17

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

Published on: May 10, 2024

1.2K

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Biophysics

Background:

  • CRISPR-Cas systems (Cas12a, Cas13a) are RNA-guided endonucleases for specific nucleic acid detection.
  • Existing methods often require labels and can be complex.
  • Nucleic acid detection is crucial for diagnostics, pathogen identification, and genotyping.

Purpose of the Study:

  • To develop a label-free detection method for specific nucleic acid sequences.
  • To utilize liquid-liquid phase separation (LLPS) for nucleic acid detection.
  • To leverage CRISPR-Cas enzymes with LLPS for a simplified diagnostic assay.

Main Methods:

  • Employed CRISPR-Cas12a and Cas13a enzymes for target recognition.
  • Utilized liquid-liquid phase separation (LLPS) driven by polymer charge interactions.
  • Measured detection via solution turbidity changes caused by LLPS.
  • Validated the Voorn-Overbeek model for LLPS in polynucleotide/polycation mixtures.

Main Results:

  • Demonstrated label-free, sequence-specific detection of nucleic acids using CRISPR-Cas and LLPS.
  • Observed naked-eye detection of nucleic acids due to LLPS-induced turbidity.
  • Identified an optimal polynucleotide concentration for enhanced detection sensitivity.
  • Provided theoretical predictions for improving LLPS-based detection assays.

Conclusions:

  • LLPS combined with CRISPR-Cas enzymes offers a simple, low-cost method for nucleotide sequence detection.
  • This approach complements existing CRISPR-based diagnostics, enhancing accessibility.
  • The turbidity readout provides a visual and sensitive detection mechanism.