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Genetic glycoengineering in mammalian cells.
Yoshiki Narimatsu1, Christian Büll2, Yen-Hsi Chen3
1Department of Cellular and Molecular Medicine, Faculty of Health Sciences, Copenhagen Center for Glycomics, University of Copenhagen, Copenhagen, Denmark; GlycoDisplay ApS, Copenhagen, Denmark.
The Journal of Biological Chemistry
|February 22, 2021
Summary
Nuclease-based gene editing allows precise genetic engineering of mammalian cell glycosylation. This advances glycomics research and therapeutic development by enabling stable glycan modification and single-cell analysis.
Area of Science:
- Biochemistry
- Cell Biology
- Genetics
Background:
- Glycosylation is crucial for cellular functions and therapeutic glycoproteins.
- Traditional glycoengineering methods have limitations in precision and stability.
- Nuclease-based gene editing offers new possibilities for glycan manipulation.
Purpose of the Study:
- To review the current state of genetic glycoengineering in mammalian cells.
- To highlight emerging opportunities and applications in glycosciences.
- To discuss the impact of gene editing on glycome research and biomedicine.
Main Methods:
- Utilizing nuclease-based gene-editing technologies for precise genetic modification.
- Systematic engineering of glycosylation capacities in mammalian cell lines.
- Integration of glycoengineering data into omics workflows.
Main Results:
- Precise and stable genetic engineering of glycosylation is now achievable.
- Glycoengineering enables exploration of glycan functions at the single-cell level.
- New applications have led to discoveries in glycan function and improved therapeutics.
Conclusions:
- Genetic glycoengineering in mammalian cells is a rapidly advancing field.
- Precise gene editing provides a powerful platform for glycome exploration.
- Emerging opportunities promise significant advancements in basic and applied glycosciences.
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