Related Experiment Video

Updated: Nov 16, 2025

Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane
05:36

Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane

Published on: February 8, 2020

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CircAGAP1 promotes tumor progression by sponging miR-15-5p in clear cell renal cell carcinoma

Qi Lv1, Gangmin Wang2, Yinan Zhang3

  • 1Department of Medical Imaging, Tongji Hospital, Tongji University School of Medicine, Xincun Road No. 389, Shanghai, China.

Journal of Experimental & Clinical Cancer Research : CR
|February 23, 2021
PubMed
Abstract

Insights

Circular RNA AGAP1 (circAGAP1) is upregulated in clear cell renal cell carcinoma (ccRCC), promoting tumor growth by sponging miR-15-5p and increasing E2F3 expression. Targeting circAGAP1 offers a potential therapeutic strategy for ccRCC.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are emerging as key regulators in cancer development.
  • The specific roles of circRNAs in clear cell renal cell carcinoma (ccRCC) remain largely unexplored.

Purpose of the Study:

  • To investigate the expression, function, and molecular mechanism of circAGAP1 in ccRCC.
  • To explore the potential of circAGAP1 as a therapeutic target in ccRCC.

Main Methods:

  • Quantitative analysis of circAGAP1 expression in ccRCC tissues and cell lines.
  • In vitro and in vivo functional assays to determine circAGAP1's role.
  • Molecular mechanism studies including RNA immunoprecipitation and luciferase assays to elucidate interactions with miR-15-5p and E2F3.

Main Results:

  • circAGAP1 expression is significantly upregulated in ccRCC and correlates with tumor progression.
  • circAGAP1 acts as a molecular sponge for miR-15-5p, leading to decreased miR-15-5p levels.
  • circAGAP1 promotes ccRCC progression by upregulating E2F3 expression via sponging miR-15-5p.

Conclusions:

  • circAGAP1 functions as an oncogenic circRNA in ccRCC through the miR-15-5p/E2F3 axis.
  • circAGAP1 may serve as a novel biomarker and therapeutic target for ccRCC.
Keywords:
Clear cell renal carcinomaE2F3ceRNAcircRNAmiR-15

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