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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Nov 16, 2025

Sample Preparation for Mass Cytometry Analysis

Sample Preparation for Mass Cytometry Analysis

Published on: April 29, 2017

Research Techniques Made Simple: Experimental Methodology for Imaging Mass Cytometry.

Sheida Naderi-Azad¹, David Croitoru², Saeed Khalili²

¹Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada.

The Journal of Investigative Dermatology

|February 23, 2021

Summary

This summary is machine-generated.

Imaging mass cytometry (IMC) advances protein analysis by visualizing 40+ cellular markers in situ. This technique preserves tissue architecture, enabling detailed study of cellular interactions in dermatology research.

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Related Experiment Videos

Last Updated: Nov 16, 2025

Sample Preparation for Mass Cytometry Analysis

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Published on: April 29, 2017

Mass Cytometry: Protocol for Daily Tuning and Running Cell Samples on a CyTOF Mass Cytometer

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Area of Science:

Biotechnology
Cellular Biology
Immunology

Background:

Flow cytometry and mass cytometry by time-of-flight (CyTOF) have expanded multi-analyte measurements.
Recent advancements include imaging mass cytometry (IMC), a powerful imaging technique.

Purpose of the Study:

To review the experimental methodology of imaging mass cytometry (IMC).
To highlight IMC's capability in visualizing cellular markers and preserving tissue architecture for in situ analysis.

Main Methods:

IMC utilizes rare metal isotopes conjugated to antibodies for high-resolution imaging.
The technique involves in situ staining and direct tissue vaporization.
A 2D spectral reconstruction is generated from CyTOF-captured events.

Main Results:

IMC allows visualization of up to 40 unique cellular markers.
Preserves two-dimensional (2D) tissue architecture.
Enables high-resolution, multilayer images of protein expression, cellular localization, and interactions.

Conclusions:

IMC is a valuable tool for in situ analysis in dermatology research.
It provides detailed insights into cellular behavior within tissue context.
The methodology facilitates a deeper understanding of biological processes at the cellular level.