A method for intracellular injection of horseradish peroxidase by pressure

A technique for intracellular injection of horseradish peroxidase and other tracers into neurones is described. The method utilises gas pressure to force the tracer solution out of glass micropipettes and allows electrophysiological recordings to be made simultaneously with the injection process. Constructional details of the simple and inexpensive equipment are given. The method has the advantages of being equally suitable for charged and uncharged tracer molecules, and of providing reliable indication of the success of the injection at the time of the injection attempt.