Lysobacter penaei sp. nov., isolated from intestinal content of a Pacific white shrimp (Penaeus vannamei)

The polyphasic taxonomic approach was used to characterize a novel bacteria strain, designated SG-8^T, which was isolated from intestinal content of a Pacific white shrimp (Penaeus vannamei). Cells were Gram-stain-negative, aerobic, non-gliding rods. Growth occurred at 10-45 °C (optimum, 20-30 °C), pH 5.0-10.0 (optimum, 6.0-7.0) and in 0-6.0 % (w/v) NaCl (optimum, 0-4.0 %). The 16S rRNA gene sequence of strain SG-8^T showed the highest sequence similarity to Lysobacter maris KMU-14^T (98.6 %). On phylogenetic trees, strain SG-8^T formed a stable cluster with Lysobacter maris KMU-14^T, Lysobacter alkalisoli SJ-36^T, Lysobacter spongiae 119BY6-57^T and Lysobacter aestuarii S2-C^T. The average nucleotide identity and digital DNA-DNA hybridization values between strain SG-8^T and the four reference type strains listed above were 83.3, 82.3, 83.5, 83.3% and 22.8, 22.7, 22.7, 22.9 %, respectively. The major fatty acids (>5 %) were iso-C_15 : 0, summed feature 9 (iso-C_17 : 1  ω9c and/or 10-methyl C_16 : 0), iso-C_16 : 0, summed feature 3 (C_16 : 1  ω6c and/or C_16 : 1  ω7c), iso-C_17 : 0, iso-C_11 : 0 3OH and iso-C_11 : 0. Ubiquinone-8 (Q-8) was the only respiratory quinone. The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The DNA G+C content was 68.8 mol%. Based on the results of genomic, phylogenetic, phenotypic and chemotaxonomic analyses, strain SG-8^T represents a novel species of the genus Lysobacter, for which the name Lysobacter penaei sp. nov. is proposed. The type strain is SG-8^T (=GDMCC 1.1817^T=KACC 21942^T).