Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Planning the suicide experiments.

S Hazout, A J Valleron

    Cell and Tissue Kinetics
    |November 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    This study uses statistical methods to improve the precision of suicide experiments. We determined the optimal number of experimental units needed for accurate cell analysis and population comparisons.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Association of environmental markers with childhood type 1 diabetes mellitus revealed by a long questionnaire on early life exposures and lifestyle in a case-control study.

    BMC public health·2016
    Same author

    Extraction of melodies in behavioural sequences.

    Behavioural processes·2014
    Same author

    Estimating influenza latency and infectious period durations using viral excretion data.

    Epidemics·2012
    Same author

    Real-time comparative monitoring of the A/H1N1 pandemic in France.

    Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2010
    Same author

    The rising impact of mathematical modelling in epidemiology: antibiotic resistance research as a case study.

    Epidemiology and infection·2007
    Same author

    "Pinning strategy": a novel approach for predicting the backbone structure in terms of protein blocks from sequence.

    Journal of biosciences·2007
    Same journal

    Haemopoietic stem cells: spleen colony-forming cells are normally actively proliferating.

    Cell and tissue kinetics·1990
    Same journal

    Proliferative changes in the genital tissue of female mice during the oestrous cycle.

    Cell and tissue kinetics·1990
    Same journal

    Lack of correlation between thymidine kinase activity and changes of DNA synthesis with tumour age: an in vivo study in Ehrlich ascites tumour.

    Cell and tissue kinetics·1990
    Same journal

    Effects of vitamin A deficiency on cell proliferation and morphology of trachea of the hamster.

    Cell and tissue kinetics·1990
    Same journal

    New methods for calculating kinetic properties of cells in vitro using pulse labelling with bromodeoxyuridine.

    Cell and tissue kinetics·1990
    Same journal

    Disruption of microtubules induces an endogenous suicide pathway in human leukaemia HL-60 cells.

    Cell and tissue kinetics·1990
    See all related articles

    Area of Science:

    • Cell biology
    • Statistical analysis
    • Biostatistics

    Background:

    • Suicide experiments often face uncertainty in cell colony counting.
    • Accurate estimation of DNA synthesizing cells (S cells) is crucial for biological studies.

    Purpose of the Study:

    • To statistically analyze the precision of cell colony estimation in suicide experiments.
    • To determine the required number of experimental units for accurate S cell percentage determination.
    • To provide methods for testing cell population quiescence and comparing S cell percentages between populations.

    Main Methods:

    • Statistical modeling of colony numbers assuming a Poisson distribution.
    • Calculation of the necessary number of recipients for desired accuracy levels.
    • Application of statistical tests for population quiescence and comparison.

    Related Experiment Videos

    Main Results:

    • Provides a framework for calculating the required number of experimental units based on desired precision.
    • Offers statistical methods to address uncertainty in suicide experiment colony counts.
    • Enables robust testing of cell population quiescence and comparative S cell analysis.

    Conclusions:

    • Statistical analysis enhances the precision of suicide experiments.
    • The number of experimental units directly impacts the accuracy of S cell percentage estimation.
    • This work provides a statistically sound approach for analyzing cell populations in suicide assays.