Circular RNA circ_0002137 regulated the progression of osteosarcoma through regulating miR-433-3p/ IGF1R axis

Area of Science:

• Oncology
• Molecular Biology
• Genetics

Background:

• Current treatments for osteosarcoma (OS) are insufficient for patients with metastasis or relapse, leading to high mortality.
• Comprehensive molecular mechanisms underlying OS development remain largely unknown.
• Circular RNAs (circRNAs) are increasingly recognized for their roles in cancer progression.

Purpose of the Study:

• To investigate the role of circRNA hsa_circ_0002137 in osteosarcoma (OS) development and progression.
• To elucidate the molecular mechanism by which hsa_circ_0002137 influences OS.
• To identify potential therapeutic targets for advanced OS.

Main Methods:

• Expression analysis of hsa_circ_0002137 in OS tissues and cell lines.
• Functional experiments involving down-regulation and up-regulation of hsa_circ_0002137.
• Bioinformatic prediction, RNA pull-down, and luciferase reporter assays to verify interactions.
• Investigation of the regulatory axis involving hsa_circ_0002137, miR-433-3p, and insulin-like growth factor-1 receptor (IGF1R).

Main Results:

• hsa_circ_0002137 was significantly upregulated in osteosarcoma tissues and cell lines.
• Down-regulation of hsa_circ_0002137 suppressed OS cell invasion, cell cycle progression, and induced apoptosis.
• hsa_circ_0002137 was confirmed to interact with miR-433-3p and regulate IGF1R expression.
• The inhibitory effect of hsa_circ_0002137 on OS progression was dependent on the miR-433-3p/IGF1R axis.

Conclusions:

• hsa_circ_0002137 acts as a tumor promoter in osteosarcoma (OS).
• hsa_circ_0002137 restrains OS progression by regulating the miR-433-3p/IGF1R axis.
• hsa_circ_0002137 represents a potential therapeutic target for osteosarcoma.