Related Experiment Video
Updated: Nov 16, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
Published on: April 11, 2019
History of genome editing: From meganucleases to CRISPR
Simon E Tröder1, Branko Zevnik1
1in vivo Research Facility, Faculty of Medicine and University Hospital Cologne, University of Cologne, Cologne, Germany.
Abstract:
CRISPR-mediated genome editing has undoubtedly revolutionized genetic engineering of animals. With the ability for virtually unlimited modification of almost any genome it is easy to forget which amazing discoveries paved the way for this ground-breaking technology. Here, we summarize the history of genome editing platforms, starting from enhanced integration of foreign DNA by meganuclease-mediated double-strand breaks to CRISPR/Cas9, the leading technology to date, and its re-engineered variants.
Related Concept Videos
CRISPR/Cas9 Genome Editing
CRISPR
CRISPR and crRNAs
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
Homologous Recombination
What is Genetic Engineering?
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...

