Sex-specific Changes in Brain Estrogen Metabolism Induced by Acute Trimethyltin Exposure
Jung Ho Lee1, Sung-Hee Cho2, Eun Hye Jang1
1Department of Pharmacology, School of Medicine, Eulji University, Daejeon, Republic of Korea.
Background/Aim:
In this study, we investigated sex-specific effects of acute exposure to trimethyltin, a known neurotoxicant on metabolic steroids.
Materials And Methods:
We administered intraperitoneally 2.3 mg/kg trimethyltin to 4-week-old male mice and measured the levels of metabolic steroids 24 h after treatment. We also measured mRNA and protein levels of cytochrome P450 1B1 using real-time polymerase chain reaction and western blotting.
Results:
Cortisol levels in the cortex increased in both sexes following acute trimethyltin exposure. The estradiol levels decreased, and the 4-hydroxyestradiol levels increased only in females. We also observed increased cytochrome P450 1B1 mRNA and protein levels only in the female cortex.
Conclusion:
Acute trimethyltin exposure induces distinct sex-specific metabolic changes in the brain before significant sexual maturation.
