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Updated: Nov 16, 2025

Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing
Published on: July 5, 2019
Weak-evidence Fusion Candidates Detected by a FusionPlex Assay Using the Ion Torrent System
Shiro Fujita1,2, Katsuhiro Masago3, Eiichi Sasaki3
1Department of Pathology and Molecular Diagnostics, Aichi Cancer Center, Nagoya, Japan; jp.shirofujita@gmail.com.
Background/Aim:
The Archer FusionPlex platform is widely used for comprehensive fusion-gene detection in cancer tissues. This platform separately displays results for strong-evidence and weak-evidence fusion candidates (WEFCs). Distinctive fusion patterns are frequently found in the weak-evidence category and information about the patterns is clinically essential.
Patients And Methods:
We describe the type and frequency of WEFCs observed using the FusionPlex Sarcoma Panel (S Panel) and the FusionPlex ALK, RET, and ROS1 ver2 Panel (ARR Panel).
Results:
A total of 97 specimens were examined and 620 candidates were detected and categorized as WEFCs. A median of five WEFCs were detected per sample. In the S Panel group, there were 13 WEFCs with a frequency of more than 1%. In the ARR Panel group, a total of 16 WEFCs were detected with a frequency of more than 1%.
Conclusion:
Specific WEFCs were detected according to the panel selected.
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