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Increasing hydrogen supply in biological biogas upgrading converts carbon dioxide to biomethane. Higher hydrogen ratios significantly boost methane content to 90%, enhancing energy recovery from sludge treatment.

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Area of Science:

  • Environmental Microbiology
  • Biotechnology
  • Renewable Energy

Background:

  • Biological biogas upgrading enhances methane yield from sources like sewage sludge.
  • Hydrogenotrophic methanogenesis utilizes hydrogen (H2) to convert carbon dioxide (CO2) into methane.
  • Optimizing H2 supply is crucial for efficient *in situ* biogas enrichment.

Purpose of the Study:

  • To investigate the impact of varying hydrogen supply on *in situ* biological biogas upgrading.
  • To monitor process performance and microbial community shifts during CO2 conversion to biomethane.
  • To determine the optimal hydrogen to carbon dioxide ratio for maximizing methane content.

Main Methods:

  • Continuous operation of two parallel reactors fed with sewage sludge over 211 days.
  • Progressive increase of the H2/CO2 molar ratio from 0.5:1 to 7:1.
  • High-throughput sequencing of 16S rRNA genes to analyze microbial community evolution.

Main Results:

  • Biogas composition changed significantly above the stoichiometric H2/CO2 ratio of 4:1.
  • A H2/CO2 ratio of 7:1 resulted in 90% methane content in biogas.
  • Organic matter degradation was not negatively impacted by increased hydrogen supply.
  • Dominance of hydrogenotrophic methanogens, specifically *Methanolinea* and *Methanobacterium*, was confirmed.

Conclusions:

  • Sufficient hydrogen supply is key to achieving high methane yields in biological biogas upgrading.
  • The process can be optimized to enrich specific hydrogenotrophic methanogens for efficient CO2 conversion.
  • This study demonstrates the feasibility of enhancing energy recovery from sludge treatment through optimized biogas upgrading.