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Updated: Nov 16, 2025

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
Comparison of mitochondrial genomes from multi-, Bi-, and uninucleate Rhizoctonia
Cheng Li1, Shanyue Zhou2, Manman Zhang1
1Key Laboratory of Pest Monitoring and Green Management, MOA, Department of Plant Pathology, China Agricultural University, Beijing, China.
Abstract:
Six circular mitochondrial genomes of multi-, bi-, and uninucleate Rhizoctonia isolates were assembled and found that all the genomes contain 14 conserved protein-coding genes, one ribosomal protein (rps3), and 23 tRNA in the same order. The mitogenome sizes of uninucleate isolates were relatively smaller than binucleate and multinucleate stains. The size variations between uninucleate and multinucleate isolates were from both intergenic and intronic regions, whereas the differences between uninucleate and binucleate isolates were predominantly from intergenic regions. The phylogenetic analysis revealed that Rhizoctonia strains of the same nucleate types had a closer relationship.
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