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MiR-34a-5p directly targeting TRIM44 affects the biological behavior of ovarian cancer cells
1Department of Gynecology, Dongying People's Hospital, Dongying, China. Duanyanan258@sina.com.
Objective:
This research intended to explore the expression and molecular mechanism of miR-34a-5p and Tripartite motif-containing protein 44 (TRIM44) in ovarian cancer (OC).
Patients And Methods:
Tissue and serums of OC patients were collected, and miR-34a-5p and TRIM44 in serum and tissue were tested by Real-time quantitative PCR (qRT-PCR). In vitro cell experiment was constructed. Methyl Thiazolyl Tetrazolium (MTT), transwell, and flow cytometry were applied to test the proliferation, migration, invasion, and apoptosis. Western blot was performed to explore TRIM44 and epithelial-mesenchymal transition (EMT) proteins.
Results:
MiR-34a-5p was low expressed, while TRIM44 was highly expressed, which was related to Federation International of Gynecology and Obstetrics (FIGO) staging and lymph node metastasis. The receiver operating characteristic curve (ROC) revealed that the Area Under Curve (AUC) of miR-34a-5p and TRIM44 were 0.885 and 0.868, respectively. An evident increase of miR-34a-5p or decrease of TRIM44 could inhibit OC malignant behaviors, and E-cadherin increased while N-cadherin and Fibronectin decreased. The knockdown of miR-34a-5p or overexpression of TRIM44 could inhibit the malignant behavior of ovarian cancer cells, hinder the malignant behaviors, and reduce E-cadherin, while N-cadherin and Fibronectin protein was enhanced. Co-transfection found that overexpression of miR-34a-5p eliminated the biological behaviors of OC cells by TRIM44.
Conclusions:
MiR-34a-5p and TRIM44 can be used as diagnostic markers for OC. MiR-34a-5p can act in biological behaviors by targeting TRIM44 in OC.
Insights
MicroRNA-34a-5p (miR-34a-5p) is downregulated while Tripartite motif-containing protein 44 (TRIM44) is upregulated in ovarian cancer (OC). MiR-34a-5p targets TRIM44 to inhibit OC progression and can serve as a diagnostic marker.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Ovarian cancer (OC) remains a significant global health challenge with complex molecular underpinnings.
- Identifying novel biomarkers and therapeutic targets is crucial for improving OC diagnosis and treatment outcomes.
Purpose of the Study:
- To investigate the expression patterns of miR-34a-5p and Tripartite motif-containing protein 44 (TRIM44) in ovarian cancer.
- To elucidate the molecular mechanism and regulatory relationship between miR-34a-5p and TRIM44 in OC progression.
Main Methods:
- Quantitative real-time PCR (qRT-PCR) was used to measure miR-34a-5p and TRIM44 levels in patient tissues and serum.
- In vitro assays including MTT, Transwell, and flow cytometry assessed cell proliferation, migration, invasion, and apoptosis.
- Western blot analysis evaluated TRIM44 and epithelial-mesenchymal transition (EMT) markers.
Main Results:
- MiR-34a-5p was significantly downregulated, while TRIM44 was upregulated in OC tissues and serum, correlating with FIGO stage and lymph node metastasis.
- Receiver operating characteristic (ROC) analysis indicated high diagnostic potential for both miR-34a-5p (AUC=0.885) and TRIM44 (AUC=0.868).
- Upregulation of miR-34a-5p or downregulation of TRIM44 inhibited OC cell proliferation, migration, and invasion, while promoting apoptosis and reversing EMT markers (increased E-cadherin, decreased N-cadherin/Fibronectin).
Conclusions:
- MiR-34a-5p and TRIM44 demonstrate potential as diagnostic biomarkers for ovarian cancer.
- MiR-34a-5p exerts its tumor-suppressive effects in OC by targeting and downregulating TRIM44, highlighting a novel therapeutic pathway.
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