Related Experiment Video

Updated: Nov 16, 2025

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR
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MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR

Published on: May 16, 2012

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Circ_0004417 inhibits the progression of prostate cancer through sponging miR-1228

H-Y Xia1, C-D Liu, W Liang

  • 1Department of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China. gswxw@njmu.edu.cn.

European Review for Medical and Pharmacological Sciences
|February 25, 2021
PubMed
Abstract

Insights

Circular RNA (circRNA) circ_0004417 is downregulated in prostate cancer (PC). Overexpression of circ_0004417 inhibits PC progression by sponging miR-1228, suggesting it as a potential therapeutic target.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are implicated in tumorigenesis and cancer progression.
  • The specific role and mechanisms of circRNAs in prostate cancer (PC) require further elucidation.

Purpose of the Study:

  • To investigate the role of circ_0004417 in prostate cancer progression.
  • To explore the underlying molecular mechanisms of circ_0004417 in PC.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) for expression analysis.
  • In vitro assays (CCK-8, colony formation, Transwell) to assess functional impact.
  • Investigation of regulatory interactions with miRNA, p-Akt, and E-cadherin.

Main Results:

  • Circ_0004417 expression was significantly downregulated in PC tissues and cell lines.
  • Overexpression of circ_0004417 inhibited PC cell proliferation and invasion.
  • Circ_0004417 acts as a sponge for miR-1228, regulating p-Akt and E-cadherin expression.

Conclusions:

  • Circ_0004417 inhibits prostate cancer progression via sponging miR-1228.
  • Circ_0004417 presents a potential therapeutic target for prostate cancer treatment.

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