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LncRNA DNAJC3-AS1 functions as oncogene in renal cell carcinoma via regulation of the miR-27a-3p/PRDM14 axis

X-Y Na1, X-Q Hu, Y Zhao

  • 1Department of Neurology, People's Hospital of Zhenhai District, Ningbo, China. shangxs320@163.com.

European Review for Medical and Pharmacological Sciences
|February 25, 2021
PubMed
Abstract

Insights

Long noncoding RNA DNAJC3-AS1 is upregulated in clear cell renal cell carcinoma (ccRCC). Silencing this lncRNA inhibits ccRCC progression by affecting cell proliferation, migration, invasion, and apoptosis via the miR-27a-3p/PRDM14 pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Renal cell carcinoma (RCC) is a prevalent urological malignancy with unsatisfactory prognosis despite diagnostic and therapeutic advancements.
  • Long noncoding RNAs (lncRNAs) are increasingly recognized for their critical roles in cancer initiation and progression.
  • Clear cell renal cell carcinoma (ccRCC) is the most common subtype of RCC, necessitating further understanding of its underlying molecular mechanisms.

Purpose of the Study:

  • To investigate the expression and functional significance of lncRNA DNAJC3-AS1 in the tumorigenesis of ccRCC.
  • To elucidate the molecular interactions involving DNAJC3-AS1, microRNA-27a-3p (miR-27a-3p), and PR domain containing 14 (PRDM14) in ccRCC.

Main Methods:

  • Quantitative real-time PCR (RT-PCR) to assess DNAJC3-AS1 expression in ccRCC tissues and cell lines.
  • In vitro experiments involving silencing DNAJC3-AS1 to evaluate its effects on ccRCC cell proliferation, migration, invasion, and apoptosis.
  • Dual-luciferase reporter assays to confirm the interaction between DNAJC3-AS1, miR-27a-3p, and PRDM14.
  • Western blotting to measure protein expression levels.

Main Results:

  • DNAJC3-AS1 expression was significantly upregulated in ccRCC tissues and cell lines compared to normal controls.
  • Silencing DNAJC3-AS1 inhibited ccRCC cell proliferation, migration, and invasion, while promoting apoptosis.
  • DNAJC3-AS1 functions as a molecular sponge for miR-27a-3p, and PRDM14 was identified as a downstream target of miR-27a-3p.

Conclusions:

  • LncRNA DNAJC3-AS1 acts as an oncogene in ccRCC development.
  • The oncogenic role of DNAJC3-AS1 in ccRCC is mediated through the regulation of the miR-27a-3p/PRDM14 axis.
  • Targeting the DNAJC3-AS1/miR-27a-3p/PRDM14 pathway may offer a novel therapeutic strategy for ccRCC.

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