Recent progress of microfluidics in surface-enhanced Raman spectroscopic analysis

Surface-enhanced Raman spectroscopy is a significant analytical tool capable of fingerprint identification of molecule in a rapid and ultrasensitive manner. However, it is still hard to meet the requirements of practical sample analysis. The introduction of microfluidics can effectively enhance the performance of surface-enhanced Raman spectroscopy in complex sample analysis including reproducibility, selectivity, sensitivity, and speed. This review summarizes the recent progress of microfluidics in surface-enhanced Raman spectroscopic analysis through four combination approaches. First, microfluidic synthetic techniques offer uniform nano-/microparticle fabrication approaches for reproductive surface-enhanced Raman spectroscopic analysis. Second, the integration of microchip and surface-enhanced Raman spectroscopic substrate provides advanced devices for sensitive and efficient detection. Third, microfluidic sample preparations enable rapid separation and preconcentration of analyte prior to surface-enhanced Raman spectroscopic detection. Fourth, highly integrated microfluidic devices can be employed to realize multistep surface-enhanced Raman spectroscopic analysis containing material fabrication, sample preparation, and detection processes. Furthermore, the challenges and outlooks of the application of microfluidics in surface-enhanced Raman spectroscopic analysis are discussed.