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Recurrent Escherichia coli Urinary Tract Infection Triggered by Gardnerella vaginalis Bladder Exposure in Mice
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Uropathogenic E. coli induces DNA damage in the bladder.

Camille V Chagneau1, Clémence Massip1,2, Nadège Bossuet-Greif1

  • 1IRSD, INSERM, Université de Toulouse, INRA, ENVT, UPS, Toulouse, France.

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Colibactin, a genotoxin produced by E. coli, was found in over 20% of human urinary tract infections (UTIs). This bacterial toxin causes DNA damage in bladder cells, contributing to UTI pathogenesis.

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Area of Science:

  • Microbiology
  • Urology
  • Genetics

Background:

  • Urinary tract infections (UTIs) are common, affecting 60% of women.
  • Uropathogenic Escherichia coli (E. coli) is a primary cause of UTIs.
  • Bacterial genotoxins may play a role in UTI development and severity.

Purpose of the Study:

  • To investigate the presence and role of colibactin in community-acquired UTIs.
  • To assess the genotoxicity of colibactin in human bladder cells.
  • To understand the expression of colibactin during early UTI stages.

Main Methods:

  • Analysis of urine samples from 223 UTI patients.
  • Isolation and characterization of E. coli strains.
  • Murine model of UTI to study bacterial genotoxin production.
  • Assessment of DNA damage in bladder cells.

Main Results:

  • Colibactin's cleavage product was detected in 55 out of 223 (24.7%) UTI patient samples.
  • Uropathogenic E. coli strains, including UTI89, were confirmed to produce colibactin.
  • Colibactin production machinery was active during early UTI in a murine model.
  • Significant DNA damage was observed in bladder cells.

Conclusions:

  • This study provides the first evidence of colibactin production in human UTIs.
  • Colibactin genotoxicity contributes to bladder cell damage during UTIs.
  • Understanding colibactin's role may lead to new UTI prevention and treatment strategies.