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A fast, high-affinity fluorescent serotonin biosensor engineered from a tick lipocalin
Shen Zhang1,2,3, Xinyu Li1,2, Shengyu Zhao1,3
1Center for Membrane and Cell Physiology, University of Virginia, Charlottesville, VA, USA.
Nature Methods
|February 26, 2021
Abstract:
Serotonin (5-HT) is an important signaling monoamine and neurotransmitter. We report structure-guided engineering of a green fluorescent, genetically encoded serotonin sensor (G-GESS) from a 5-HT-binding lipocalin in the soft tick Argas monolakensis. G-GESS shows fast response kinetics and high affinity, specificity, brightness and photostability. We used G-GESS to image 5-HT dynamics in cultured cells, brain slices and behaving mice.
