A novel modified thaw-siphon method for extracting cryoprecipitate: The Bokutoh-siphon method

Area of Science:

• Transfusion Medicine
• Hematology
• Biotechnology

Background:

• Cryoprecipitate (CRYO) is not produced by the Japanese Red Cross Society.
• A novel in-house method for CRYO extraction was developed.
• The method modifies a thaw-siphon technique.

Purpose of the Study:

• To evaluate a novel in-house cryoprecipitate extraction method.
• To compare the novel method with a standard centrifugation technique.
• To analyze differences in fibrinogen, clotting factors, and clotting activity.

Main Methods:

• Fresh frozen plasma was divided into two bags for CRYO extraction.
• CRYO was extracted using a standard centrifugation method and a modified thaw-siphon method (Bokutoh-siphon method).
• Extracted CRYOs were analyzed for fibrinogen recovery, clotting factor yield, and clotting activity.

Main Results:

• Fibrinogen recovery was similar between the B-siphon method (71.2%) and the standard method (61.0%).
• Clotting factor XIII yield was significantly lower with the B-siphon method.
• Clotting factors II, V, and C1q esterase inhibitor were significantly higher in CRYO from the B-siphon method.

Conclusions:

• A new in-house CRYO preparation method was successfully established.
• The modified thaw-siphon method yields a coagulation factor-rich CRYO.
• This method allows CRYO extraction without a large-capacity refrigerated centrifuge.