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Point-of-Care Monitoring of Colitis Using Intestinal Alkaline Phosphatase in Inflammatory Bowel Disease
Xiaolin Sheng1, Dandan Liu1, Sujani K Gamage1
1Department of Chemistry, Georgia State University 788 Petit Science Center, Atlanta, Georgia 30302, United States.
ACS Sensors
|February 26, 2021
Summary
Intestinal Alkaline Phosphatase (IAP) shows promise as a biomarker for monitoring colitis in Inflammatory Bowel Disease (IBD). A rapid Point-Of-Care assay using a glucose meter can detect IAP levels, aiding in colitis assessment.
Area of Science:
- Biochemistry
- Gastroenterology
- Biomarker Discovery
Background:
- Inflammatory Bowel Disease (IBD) management requires reliable biomarkers.
- Current monitoring methods for colitis can be invasive or time-consuming.
- Intestinal Alkaline Phosphatase (IAP) has been explored for its potential role in gut health.
Purpose of the Study:
- To investigate Intestinal Alkaline Phosphatase (IAP) as a biomarker for monitoring colitis.
- To develop a rapid, Point-Of-Care (POC) assay for IAP detection.
- To assess IAP levels in an Interleukin 10 deficient (IL 10-/-) mouse model of IBD.
Main Methods:
- Developed a novel POC assay utilizing a glucose meter to detect IAP.
- Synthesized a paracetamol-bearing compound cleaved by IAP to release detectable paracetamol.
- Analyzed fecal IAP levels in IL 10-/- mice with varying degrees of colitis and in response to anti-TNF-α treatment.
Main Results:
- Fecal IAP levels were significantly lower in mice with severe colitis compared to mild colitis.
- Mice treated with anti-Tumor Necrosis Factor-alpha (anti-TNF-α) showed substantially higher IAP levels than untreated mice (2.80 U vs 0.11 U, P < 0.0001).
- The POC assay demonstrated rapid (15 min) and user-friendly detection of IAP.
Conclusions:
- IAP is a potential biomarker for monitoring colitis severity in IBD.
- A POC glucometer-based assay for IAP offers a rapid and accessible method for assessing colitis and inflammation.
- This assay could improve the management of IBD by enabling timely monitoring of disease activity.
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