The potential impact of polyethylenimine on biological behavior of 3D-printed alginate scaffolds

Three-dimensional (3D) printing using biocompatible materials is a novel technology having a great potential for fabricating precise 3D scaffolds for tissue engineering. Alginate hydrogel undergoes unstable swelling and degradation properties as well as suffers from poor cell adhesion due to the lack of cell binding domains. These limit its applications in tissue engineering. In this study, 3D-printed alginate scaffolds were coated by branch polyethylenimine (PEI) to overcome the limitation of alginate because the branch PEI is a cationic polymer with a large number of active N-H groups. The results indicated that surface modification of 3D-printed alginate scaffolds using an appropriate concentration of PEI potentially promoted the fibroblast cells functions in 3D-printed alginate scaffolds, increased cell adhesion, cell proliferation and cell spreading through providing a large amount of N-H groups and increasing hydrophilicity of the surface. The degradation rate of alginate was degraded by interaction between N-H groups in PEI and -COO- groups in alginate structure and followed by the formation strong barrier layer in the interface of alginate and PEI. Therefore, ALG-PEI scaffolds can be a good candidate for tissue engineering and wound dressing applications.