A cathodic electro-fermentation system for enhancing butyric acid production from rice straw with a mixed culture

Bio-electrochemical system (BES) emerges as a versatile approach to handling environmental problems with the harvest of sustainable energy and value-added chemicals. To enhance the butyric acid production from rice straw, microbial fuel cell (MFC) and cathodic electro-fermentation (CEF) systems were constructed in this study. Inoculated with the same mixed culture, fermentative butyric acid production efficiency of the two BESs were evaluated with/without neutral red (NR) as electron mediator, respectively. It was found that the butyric acid fermentation efficiency in the MFC system was inefficient. While, the CEF system presented an evident positive effect on butyric acid production. The production and specific yield of butyric acid in the CEF system reached 5.54 g/L and 0.41 g/g, higher than that in the open circuit (OC) system by 17.37% and 28.13%, respectively. Mass percentage of butyric acid in the produced total volatile fatty acids (VFAs) was also increased from 44.74% to 52.76%. The addition of NR had no positive effect on the butyric acid production, due to the low contribution of electric current to the end-products. With the cathode potential of -0.80 V (vs Ag/AgCl), relative abundance of the butyric acid fermenting bacteria (Clostridium cluster IV and cluster XIVa) in the microbial mixture was increased from 20.25% in the OC system to 33.61% in the CEF system. This research work not only presents a novel method for enhancing butyric acid production by rice straw fermentation, but also aids an understanding of the fermentation mechanism in CEF systems.