Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development and validation of an LC-MS/MS method for measurement of porphobilinogen in urine.

Clinica chimica acta; international journal of clinical chemistry·2025
Same author

Instrument Multiplexing: Amplified Throughput for Measurement of Vitamin B1 in Whole Blood Using Parallel Liquid Chromatography Systems Interfaced to a Single Mass Spectrometer.

Clinical chemistry·2025
Same author

Analysis of Urinary Metanephrines Using Liquid Chromatography Tandem Mass Spectrometry.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Felbamate urolithiasis.

BMJ case reports·2023
Same author

A Simple, Fast, and Reliable LC-MS/MS Method for the Measurement of Homovanillic Acid and Vanillylmandelic Acid in Urine Specimens.

Methods in molecular biology (Clifton, N.J.)·2022
Same author

Quantification of 5-Hydroxyindoleacetic Acid in Urine by Ultra-performance Liquid Chromatography Tandem Mass Spectrometry.

Methods in molecular biology (Clifton, N.J.)·2022

Related Experiment Video

Updated: Nov 16, 2025

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry
08:06

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry

Published on: October 25, 2019

9.5K

Practical LC-MS/MS Method for 5-Hydroxyindoleacetic Acid in Urine.

Zlatuse D Clark1, Jeaneah M Cutler2, Elizabeth L Frank3

  • 1ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT.

The Journal of Applied Laboratory Medicine
|February 27, 2021
PubMed
Summary

A new liquid chromatography-tandem mass spectrometry (LC-MS/MS) method accurately measures 5-hydroxyindoleacetic acid (5-HIAA) in urine. This simple, cost-effective assay aids in diagnosing and managing carcinoid tumors.

More Related Videos

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
14:42

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Published on: September 23, 2021

5.4K
High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
10:17

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

10.0K

Related Experiment Videos

Last Updated: Nov 16, 2025

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry
08:06

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry

Published on: October 25, 2019

9.5K
Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
14:42

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Published on: September 23, 2021

5.4K
High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
10:17

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

10.0K

Area of Science:

  • Clinical Chemistry
  • Analytical Chemistry
  • Mass Spectrometry

Background:

  • Serotonin, produced by enterochromaffin cells, is overexpressed in carcinoid tumors.
  • 5-hydroxyindoleacetic acid (5-HIAA) is the primary urinary metabolite of serotonin.
  • 5-HIAA levels are crucial for diagnosing and monitoring carcinoid disease.

Purpose of the Study:

  • To develop and validate a "dilute-and-shoot" liquid chromatography-tandem mass spectrometry (LC-MS/MS) method.
  • To enable precise quantification of 5-HIAA in urine samples.
  • To provide a reliable diagnostic tool for carcinoid tumors.

Main Methods:

  • Urine samples were diluted and processed using an automated liquid handler.
  • Chromatographic separation was performed on a reversed-phase column.
  • Detection and quantification utilized a triple quadrupole mass spectrometer with electrospray ionization and multiple reaction monitoring.

Main Results:

  • The method demonstrated excellent linearity, accuracy, and precision (CVs 0.8%-5.4%) within a 0.5-100 mg/L range.
  • Results correlated highly with a comparative HPLC method (R=0.9876).
  • Minimal interference was observed from endogenous compounds, vitamins, medications, and drugs.

Conclusions:

  • A simple, cost-effective LC-MS/MS method for urinary 5-HIAA measurement was successfully developed.
  • This assay provides a valuable clinical tool for assessing carcinoid tumors.
  • The method offers reliable and accurate 5-HIAA quantification for patient management.