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Updated: Nov 16, 2025
Isolation and Culture of Primary Human Gingival Epithelial Cells using Y-27632
Published on: November 6, 2021
Effects of curing lights on human gingival epithelial cell proliferation
Background:
Light-emitting diode (LED) and quartz-tungsten-halogen (QTH) curing lights are used commonly in clinics. The aim of this study was to assess the effect of these lights on the proliferation of human gingival epithelial cells.
Methods:
Smulow-Glickman (S-G) cells were exposed to a VALO LED (Ultradent) or an XL3000 QTH (3M ESPE) light at 1 millimeter or 6 mm distance for 18, 39, 60, and 120 seconds. Untreated and Triton X-100 treated cells were used as controls. At 24, 48, and 72 hours after light exposure, cell proliferation was evaluated via a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.
Results:
The authors first evaluated the performances of these 2 lights. Both LED and QTH lights generated heat. The LED light generated less heat than the QTH light and could save approximately two-thirds of the curing time. When used for 18 seconds at a 6 mm distance, the LED light did not inhibit the proliferation of S-G cells. However, if the exposure time was longer (for example, 39, 60, or 120 seconds), the LED light inhibited cell proliferation. The inhibitory effect increased when the exposure time was increased to 39, 60, or 120 seconds. The QTH light did not inhibit S-G cell proliferation if the exposure time was less than 120 seconds.
Conclusions:
Prolonged exposure to a blue curing light (both LED and QTH) inhibits the proliferation of gingival epithelial cells and may cause damages to oral soft tissues.
Practical Implications:
In dental practices, a balance should be struck in consideration of curing time not only to cure the composites completely but also to minimize unnecessarily prolonged light exposure.
