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Updated: Nov 16, 2025

Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae
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Eomesodermin regulate decidual CD4+T cell function during human early pregnancy.

Lanting Chen1, Mengdie Li1, Fengrun Sun1

  • 1Laboratory for Reproductive Immunology, Hospital of Obstetrics and Gynecology, Fudan University Shanghai Medical College, Shanghai, PR China.

Journal of Reproductive Immunology
|February 27, 2021
PubMed
Summary

Eomesodermin (Eomes) expression in decidual CD4+T cells is crucial for maternal-fetal tolerance. Lower Eomes+dCD4+T cell levels and function are linked to miscarriage, suggesting Eomes as a potential therapeutic target.

Keywords:
Decidual CD4(+)T cellsEomesMiscarriagePregnancy

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Area of Science:

  • Immunology
  • Reproductive Biology
  • Cellular Biology

Background:

  • Decidual CD4+T (dCD4+T) cells are essential for maternal-fetal tolerance, and their dysfunction is linked to miscarriage.
  • The T-box transcription factor eomesodermin (Eomes) role in dCD4+T cell function during early pregnancy was investigated.

Purpose of the Study:

  • To investigate the role of eomesodermin (Eomes) in regulating decidual CD4+T (dCD4+T) cells during early pregnancy.
  • To explore the potential of Eomes+dCD4+T cells as biomarkers and therapeutic targets for miscarriage.

Main Methods:

  • Analysis of Eomes expression in dCD4+T cells from normal and miscarriage pregnancies.
  • Assessment of cytokine production (Th2, Treg) by Eomes+dCD4+T cells.
  • Evaluation of Eomes+dCD4+T cell responses to progesterone, trophoblasts, and cell lines.

Main Results:

  • Higher Eomes expression in dCD4+T cells during normal pregnancy, with Eomes+dCD4+T cells showing active status and producing more Th2/Treg cytokines.
  • Decreased numbers and altered function of Eomes+dCD4+T cells observed in miscarriage.
  • Progesterone increased Eomes expression in dCD4+T cells from miscarriage but not normal pregnancy.
  • Increased frequency of Eomes+dCD4+T cells from miscarriage responded to specific stimuli, suggesting therapeutic potential.

Conclusions:

  • Eomes plays a significant role in dCD4+T cell function, contributing to maternal-fetal tolerance.
  • Altered Eomes+dCD4+T cell dynamics are associated with miscarriage, positioning Eomes as a potential early warning marker and therapeutic target.
  • Further research is needed to confirm causality and optimize therapeutic strategies targeting Eomes.