Mannheimia bovis sp. nov., Isolated from a Dead Cow with Hemorrhagic Pneumonia

Strain ZY190616^T was isolated from lung of a dead cow with hemorrhagic pneumonia in Yunnan Province, China. The strain was Gram-stain-negative, facultatively anaerobic bacterium. Phylogenetic analysis based on 16S rRNA gene sequence indicated that the strain was closely related to species of the genus Mannheimia and formed an independent clade with M.varigena CCUG 38462 ^T (97.0% similarity). Phylogenetic analysis based on recN gene indicated that the strain formed a clade with M.caviae CCUG 59995 ^T (87.8% similarity). Phylogenetic analysis based on rpoB gene indicated that the strain formed a clade with M.varigena CCUG 38462 ^T (94.7% similarity). The genomic OrthoANI values between strain ZY190616^T and M. ovis, M.haemolytica and M.granulomatis were 84.5%, 82.7% and 81.9%, respectively. The genomic G + C content was 39.8 mol%. The predominant fatty acids (> 5%) of the strain were C_16:0, C_14:0, C_18:1ω7c, summed feature 3 (C_16:1 ω7c and/ or C_16:1ω6c) and summed feature 2 (C14:0 3OH/ C16:1 Iso). The major polar lipids were phosphatidylglycerol (PG), phosphatidylethanolamine (PE), monophosphatidylglycerol (MGDG), triacylglycerol (TAG) and diphosphatidylglycerol (DLCL). The sole respiratory quinone was CoQ-7. Based on evidence from the taxonomic study, strain ZY190616^T represents a novel species of the genus Mannheimia, for which the name Mannheimia bovis sp. nov. is proposed. The type strain is ZY190616^T (= CCTCC AB 2020168 ^T = KCTC 25018 ^T).