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Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

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Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
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Updated: Nov 16, 2025

Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects
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Engineering pest tolerance through plant-mediated RNA interference.

Seung Ho Chung1, Honglin Feng1, Georg Jander1

  • 1Boyce Thompson Institute, 533 Tower Road, Ithaca, NY 14853, USA.

Current Opinion in Plant Biology
|February 27, 2021
PubMed
Summary
This summary is machine-generated.

RNA interference (RNAi) in transgenic plants offers targeted pest control with low environmental impact. Further research is crucial to optimize RNAi efficacy and minimize resistance and off-target effects in agriculture.

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Area of Science:

  • Agricultural Science
  • Biotechnology
  • Entomology

Background:

  • RNA interference (RNAi) using transgenic plants is a novel pest control strategy.
  • RNAi offers high target specificity and reduced environmental risks compared to chemical insecticides.
  • Limited commercial application of RNAi crop traits hinders widespread adoption.

Purpose of the Study:

  • To review the potential and challenges of RNAi technology in genetically engineered plants for pest management.
  • To identify factors affecting RNAi efficacy and the development of insect resistance.
  • To highlight areas for future research to improve RNAi applications in agriculture.

Main Methods:

  • Literature review of laboratory and field studies on insect-targeted RNAi in plants.
  • Analysis of factors influencing RNAi effectiveness, including RNA degradation, uptake, and transport.
  • Examination of genetic engineering strategies for RNAi construct delivery.

Main Results:

  • Numerous studies demonstrate insect growth inhibition via RNAi in laboratory settings.
  • Variability in insect RNAi pathways impacts efficacy and resistance development.
  • Few RNAi-based genetically engineered plants have reached commercialization.

Conclusions:

  • RNAi holds significant promise for sustainable agricultural pest control.
  • Overcoming challenges in RNAi delivery, stability, and insect response is key for commercial success.
  • Further research on optimal gene targets and minimizing off-target effects is essential for advancing RNAi technology.