Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Enantioselective Radical Ring-Opening Cyanation of Oxime Esters by Dual Photoredox and Copper Catalysis.

Organic letters·2019

Same author

ACCELERATING MAGNETIC RESONANCE IMAGING VIA DEEP LEARNING.

Proceedings. IEEE International Symposium on Biomedical Imaging·2019

Same author

Technical note: Development and application of KASP assays for rapid screening of 8 genetic defects in Holstein cattle.

Journal of dairy science·2019

Same author

Sesquiterpenes and diterpenes from Euphorbia thymifolia.

Fitoterapia·2019

Same author

Glechomanamides A-C, Germacrane Sesquiterpenoids with an Unusual Δ<sup>8</sup>-7,12-Lactam Moiety from <i>Salvia scapiformis</i> and Their Antiangiogenic Activity.

Journal of natural products·2019

Same author

Parameter optimization framework on wave gradients of Wave-CAIPI imaging.

Magnetic resonance in medicine·2019

Same journal

Segmentation-guided photon pooling enables robust single-cell analysis and fast fluorescence lifetime imaging microscopy.

Journal of biomedical optics·2026

Same journal

Method of spatial scanning of modulated laser radiation for outline imaging of interphalangeal joints.

Journal of biomedical optics·2026

Same journal

Multimodal optical imaging for the assessment of the teratogenic effects of ethanol on zebrafish development.

Journal of biomedical optics·2026

Same journal

Fluorescence properties of collagen types I-V: a comprehensive study of spectral and lifetime characteristics.

Journal of biomedical optics·2026

Same journal

Spectral dependence of lipofuscin fluorescence lifetimes revealed by FLIM with a superconducting nanowire single-photon detector.

Journal of biomedical optics·2026

Same journal

Building the future of biophotonics through experiential education and seasonal schools.

Journal of biomedical optics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Nov 16, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Accelerating 3D single-molecule localization microscopy using blind sparse inpainting.

Sunil Kumar Gaire¹, Yanhua Wang², Hao F Zhang³

¹The State University of New York at Buffalo, Department of Electrical Engineering, Buffalo, New York, United States.

Journal of Biomedical Optics

|February 28, 2021

Summary

This summary is machine-generated.

Accelerating 3D super-resolution microscopy (SMLM) is crucial. This study uses computational blind sparse inpainting to reconstruct high-density 3D SMLM images from fewer frames, significantly reducing acquisition and processing times without hardware changes.

Keywords:

3D imaging image reconstruction inpainting microtubules optimization single-molecule localization microscopy super-resolution

More Related Videos

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay

Published on: September 27, 2021

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Published on: December 1, 2016

Related Experiment Videos

Last Updated: Nov 16, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay

Published on: September 27, 2021

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Published on: December 1, 2016

Area of Science:

Biophysics
Microscopy
Computational Imaging

Background:

Single-molecule localization microscopy (SMLM) achieves super-resolution imaging but requires extensive frame acquisition, leading to long imaging and processing times.
Accelerating SMLM is essential for studying dynamic biological processes at the nanoscale.

Purpose of the Study:

To develop a computational method for accelerating three-dimensional (3D) SMLM imaging.
To reduce image acquisition and data processing times without sacrificing resolution.

Main Methods:

Utilized blind sparse inpainting to reconstruct high-density 3D SMLM images from low-density datasets.
Low-density images were generated using significantly fewer frames than standard SMLM protocols.

Main Results:

Demonstrated superior reconstruction of 3D SMLM images using computational inpainting.
Achieved significant frame reduction: up to 10-fold in simulations and 50-fold in experimental data.

Conclusions:

Successfully demonstrated fast 3D SMLM imaging via a computational approach reducing acquired frames.
This technique paves the way for real-time live-cell 3D imaging of nanoscopic biological structures and functions.